Flora Library
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reducing agent host:plantpath.unl.edu
Listing 1 - 3 from 3 for reducing agent
reducing agent cracking buffer sample buffer dithiothreitol dtt phenol extraction buffer composition reduce protein silver stain tributylphosphine tbp oxidized dtt salt concentration look for trends electrophoresis buffers proteins reoxidize parallel samples nasty samples
SDS gel electrophoresis - advice for beginners
... Reducing agents Proteins are normally reduced (SS bonds broken) with a thiol in the sample buffer before electrophoresis. Mercaptoethanol (ME) is the standard reducing agent. Since it's a relatively weak reducing agent ...
plantpath.unl.edu
Stabilizing and "resurrecting" DTT (dithiothreitol) stock solutions
... aliquots from the upper phase to reduce protein samples. Principle TBP (cheap) is a stronger reducing agent that DTT and reduces oxidized DTT. TBP is less dense than water, 1-bromo-3 ...
plantpath.unl.edu
Preparing samples for SDS gel electrophoresis
... for trends Are conditions adequately reducing? Use a difficult to reduce protein such as serum albumin. Compare parallel samples with different levels of reducing agent (ME, DTT) Is your reducing agent good? Use different lots ...
plantpath.unl.edu